Page 40 - 南京医科大学自然版
P. 40
第44卷第11期
·1506 · 南 京 医 科 大 学 学 报 2024年11月
A B C
α⁃SMA/DAPI(×400) Vimentin/DAPI(×400)
12
11
10 9
adjust) 8 7 Significant
(P 6 Up(46)
NO Significance(62 492)
-lg 5
3 4 Down(165)
2
1
0
-10 -5 0 5 10 15 20 25
log2FC
D E 1.5 si⁃Ctrl
(ES) 0.1 Enrichment profile Hits Ranking metric scores siLOX⁃1
siLOX⁃2
noise) Enrichment score
-0.1 0 1.0
-0.2
-0.3 Correlated expression
-0.4 0.5
-0.5 ** ** *** ** *** ** *** ** ***
Ranked list metric 2 (Signal -2 2 0 0 5 10 15 20 25 30 35 40 0 LOX CLDN2 CD34 ITGAV PTPRF
*
*
*
* ***
*
-4
Rank in ordered dataset
A:The expression characteristics of α⁃SMA in human fibroblasts(×400). B:The expression characteristics of Vimentin in human fibroblasts(×
400). C:Volcano plot of expression difference(Padjust < 0.05,FC≥2.0). D:The GSEA enrichment analysis revealed a significant downregulation of cell
adhesion molecules in siLOX group. E:Differences of cell adhesion molecules in human fibroblasts induced by silencing LOX were analyzed using qRT⁃
***
PCR. Compared with the si⁃Ctrl group, P < 0.001(n=3).
图4 沉默LOX可显著下调细胞黏附分子表达
Figure 4 Silencing LOX significantly downregulated the expression of cell adhesion molecules
A CBP(μg/mL) C PTX(ng/mL) E PTX(ng/mL)
0 100 200 0 50 100 250 500 0 5 50 100 250 500
LOX 47 kDa LOX 47 kDa LOX 47 kDa
β⁃actin 42 kDa
GAPDH 36 kDa β⁃actin 42 kDa
B 1.5 *** D 2.5 *** *** F 6 ***
LOX mRNA expression 1.0 LOX mRNA expression 1.5 LOX mRNA expression 4 2 ***
2.0
1.0
0.5
0.5
0
Ctrl 100 200 0 Ctrl 50 100 250 500 0 Ctrl 5 50 100 250 500
CBP(μg/mL) PTX(μg/mL) PTX(μg/mL)
A:Western blot validation of differences in LOX protein expression in human fibroblasts induced by different concentrations of carboplatin. B:Dif⁃
ferences in LOX expression in human fibroblasts induced by varying concentrations of carboplatin were analyzed using RT⁃qPCR. C:Western blot vali⁃
dation of differences in LOX protein expression in human fibroblasts induced by different concentrations of paclitaxel. D:Differences in LOX expression
in human fibroblasts induced by varying concentrations of paclitaxel were analyzed using RT⁃qPCR. E:Western blot validation of differences in LOX
protein expression in L929 induced by different concentrations of paclitaxel. F:Differences in LOX expression in L929 induced by varying concentra⁃
tions of paclitaxel were analyzed using RT⁃qPCR. *** P < 0.001(n=3).
图5 PTX处理可促进成纤维细胞LOX的表达
Figure 5 PTX treatment promoted the expression of LOX in fibroblasts