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第44卷第11期
               ·1506 ·                           南 京    医 科 大 学 学         报                        2024年11月


              A                         B                        C
                     α⁃SMA/DAPI(×400)        Vimentin/DAPI(×400)
                                                                     12
                                                                     11
                                                                     10 9
                                                                    adjust)  8 7             Significant

                                                                    (P 6                      Up(46)
                                                                                              NO Significance(62 492)
                                                                    -lg 5
                                                                      3 4                     Down(165)
                                                                      2
                                                                      1
                                                                      0
                                                                      -10 -5  0  5  10 15 20 25
                                                                               log2FC
               D                                                      E   1.5  si⁃Ctrl
                   (ES)  0.1  Enrichment profile  Hits  Ranking metric scores  siLOX⁃1
                                                                               siLOX⁃2
                    noise) Enrichment score
                    -0.1 0                                                1.0
                    -0.2
                    -0.3                                                 Correlated expression
                    -0.4                                                  0.5
                    -0.5                                                       **    ** ***  ** ***  ** ***  ** ***
                  Ranked list metric  2 (Signal  -2 2 0 0  5  10  15  20  25  30  35  40  0  LOX  CLDN2  CD34  ITGAV  PTPRF
                                                                                      *
                                                                                            *
                                                                                                   *
                                                                               * ***
                                                                                                          *
                     -4
                                     Rank in ordered dataset


                 A:The expression characteristics of α⁃SMA in human fibroblasts(×400). B:The expression characteristics of Vimentin in human fibroblasts(×
              400). C:Volcano plot of expression difference(Padjust < 0.05,FC≥2.0). D:The GSEA enrichment analysis revealed a significant downregulation of cell
              adhesion molecules in siLOX group. E:Differences of cell adhesion molecules in human fibroblasts induced by silencing LOX were analyzed using qRT⁃
                                       ***
              PCR. Compared with the si⁃Ctrl group, P < 0.001(n=3).
                                            图4 沉默LOX可显著下调细胞黏附分子表达
                          Figure 4 Silencing LOX significantly downregulated the expression of cell adhesion molecules



                A        CBP(μg/mL)           C          PTX(ng/mL)            E           PTX(ng/mL)
                         0  100 200                    0  50 100 250 500                 0  5 50 100 250 500
                    LOX            47 kDa         LOX                 47 kDa        LOX                 47 kDa
                                                β⁃actin               42 kDa
                  GAPDH            36 kDa                                         β⁃actin               42 kDa
                  B   1.5     ***                D  2.5     *** ***               F    6        ***
                     LOX mRNA expression  1.0      LOX mRNA expression  1.5          LOX mRNA expression  4 2  ***
                                                    2.0

                                                    1.0
                      0.5
                                                    0.5
                       0
                          Ctrl  100 200              0  Ctrl 50 100 250 500            0  Ctrl 5  50 100 250 500
                             CBP(μg/mL)                      PTX(μg/mL)                        PTX(μg/mL)
                 A:Western blot validation of differences in LOX protein expression in human fibroblasts induced by different concentrations of carboplatin. B:Dif⁃
              ferences in LOX expression in human fibroblasts induced by varying concentrations of carboplatin were analyzed using RT⁃qPCR. C:Western blot vali⁃
              dation of differences in LOX protein expression in human fibroblasts induced by different concentrations of paclitaxel. D:Differences in LOX expression
              in human fibroblasts induced by varying concentrations of paclitaxel were analyzed using RT⁃qPCR. E:Western blot validation of differences in LOX
              protein expression in L929 induced by different concentrations of paclitaxel. F:Differences in LOX expression in L929 induced by varying concentra⁃
              tions of paclitaxel were analyzed using RT⁃qPCR.  *** P < 0.001(n=3).
                                            图5   PTX处理可促进成纤维细胞LOX的表达
                                  Figure 5 PTX treatment promoted the expression of LOX in fibroblasts
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