Objective：Preparation and characterization of a thermo⁃sensitive hydrogel consisting of hyaluronic acid(HA)，hydroxypropyl methylcellulose(HPMC)and glycerol，and loaded with antimicrobial peptide Funme(FP). Methods：HA/HPMC/Glycerol⁃FP hydrogels were synthesized by physical cross ⁃ linking，and the thermosensitive behavior of the hydrogels was analyzed. The physicochemical properties of the hydrogels were characterized by rheological test，Fourier transform infrared spectroscopy(FTIR)and scanning electron microscope(SEM). The biocompatibility and antibacterial activities were also investigated in vitro. A full⁃thickness skin defect model with a diameter of 10 mm was constructed on the back of the mouse and the wound healing rate was recorded after treatment with hydrogel. Results：HA/HPMC/Glycerol⁃FP hydrogel exhibited desirable gel temperature when the ratio of HA，HPMC and glycerol was 2∶7∶20 and no chemical reactions occurred during hydrogelation. Moreover，the hydrogel exhibited good biocompatibility on L929 cells and showed a significant inhibitory effect on Escherichia coli(E. coli). Animal experiments showed that wound healing in the HA/ HPMC/Glycerol⁃FP group was significantly better than the control group. Conclusion：HA/HPMC/Glycerol⁃FP is a conductive material that has great application prospects in bio⁃medical dressings.