Protective effect of TIR/BB⁃loop mimetic AS⁃1 on renal ischemia/reperfusion injury in mice
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R329.26

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    Abstract:

    Objective:To explore the effect and mechanism of TIR/BB⁃loop mimetic AS⁃1 on renal ischemia/reperfusion injury in mice. Methods:Male C57BL/6 mice were randomly and equally divided into four groups:sham operation group(Sham group),renal ischemia/reperfusion injury group(RIR group),AS ⁃ 1+ renal ischemia/reperfusion injury group(AS ⁃ 1+RIR group),vehilce + renal ischemia/reperfusion injury group(vehilce + RIR group). The renal ischemia/reperfusion injury model was constructed by clipping bilateral renal veins for 45 min and reperfusion for 24 h. The AS⁃1+RIR group and vehilce+RIR group were intraperitoneally injected with 50 mg/kg AS ⁃ 1 and vehicle 30 minutes before operation. After 24 h reperfusion,renal functional parameters,serum mediator concentrations,markers of apoptosis indexes and expression of phosphorylated NF⁃κB p65 were determined. Results:Serum creatinine (Scr)、blood ureanitrogen(BUN)、TNF⁃α、IL⁃6、Cleaved caspase3、Bax and p⁃NF⁃κB p65 were significantly increased after renal IR compared with the sham group(P<0.01). The expression of Bcl⁃2 and the ratio of Bcl⁃2/Bax were significantly decreased(P < 0.01). After treatment with AS⁃1,the levels of Scr、BUN、TNF⁃α、IL⁃6、cleaved caspase 3、Bax and P⁃p65 were significantly decreased(P < 0.01),the expression of Bcl ⁃2 was increased(P < 0.01),and the ratio of Bcl ⁃2/Bax was also increased. Conclusion:TIR/BB loop analogue AS ⁃ 1 has protective effect on renal ischemia ⁃ reperfusion injury in mice. The mechanism may be related to its anti ⁃ inflammatory and anti apoptotic effects by inhibiting the activation of NF ⁃ κ B signaling pathway.

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刁爱芹,张光际,王卉,潘爱萍,袁海建,李建涛. TIR/BB环拟似物AS⁃1对小鼠肾缺血再灌注损伤的保护作用研究[J].南京医科大学学报(自然科学版英文版),2022,42(8):1080-1086.

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  • Online: August 07,2022
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