Objective：This study aims to to develop rapid detection methods and establish a new method for a common foodborne pathogenic microorganism enterohemorrhagic E.coli(EHEC)O157：H7 detection. Methods：Two EHEC O157：H7 detection methods were established and optimized based on the conserved region sequence of EHEC O157：H7 combined with recombinase-aided amplification(RAA)and fluorescence quantitative method or lateral flow dipstick(LFD)，then the sensitivity and specificity of the two methods(RAA fluorescence method and RAA-LFD method)were evaluated. Results：Both detection methods can be completed within 16 min at 39 ℃，and can detect 1 × 104 copies，showing high sensitivity. The two methods did not cross react with plasmid templates of other common intestinal pathogens(Staphylococcus aureus，Salmonella，Campylobacter，Yersinia，Shigella)and showed good specificity. Conclusion：The two EHEC O157：H7 detection methods established in this study have short detection time，good specificity，sensitivity and repeatability，and can be used for rapid detection of EHEC O157：H7.