Objective：This study aims to investigate the detection rate and influencing factors of thyroid nodules(TN)among healthy teachers. Methods：The physical examination items(including gender，age，occupation，height，weight，etc.)，laboratory examination(fasting blood glucose，blood lipid，liver function，uric acid，13C breath test，etc.)and thyroid color ultrasound examination of health teachers in Sir Run Run Hospital Affiliated to Nanjing Medical University from October 2018 to June 2021 were retrospectively analyzed. Logistic regression was used to analyze the influencing factors of TN. Results：①Thyroid nodules were found in 2 539 of 6 096 patients，with a total detection rate of 41.65%，including 38.20% male and 43.03% female. There were 1 340 single nodules(21.98%)and 1 199 multiple nodules(19.67%). For TN classification according to TI-RADS，grade 3 was the most，accounting for 22.74%. The older the age，the higher the detection rate was，and there were significant differences in the detection rate of TN among different age groups(P ＜ 0.05). In addition，thyroid nodules were detected in bilateral lobe(17.27%)＞ right lobe(13.27%)＞ left lobe(10.89%)＞ thyroid isthmus(0.21%). ② The detection rate of female teachers in the middle school group was the highest(49.44%)，and there were significant differences in the detection rate of different types of teachers in the three age groups of ＜ 30 years old，30~39 years old and ≥60 years old(P ＜ 0.05)；③Compared with the non - TN group，the incidence of hypertension，abnormal fasting glucose，hyperlipidemia，overweight and obesity was higher in the TN group，and the differences were statistically significant between two groups(P ＜ 0.05). ④Logistic multifactor regression analysis showed that female was an independent risk factor for TN (OR=1.913，P ＜ 0.05)，and teachers aged 40-49，50-59 and ≥60 had a higher risk of TN than those aged less than 30(OR=1.402， 2.923，4.637，All P ＜ 0.05)，and compared with preschool education group，college teachers had a lower risk of TN(OR=0.718，P ＜ 0.05). Conclusion：The detection rate of TN among teachers who underwent physical examination is high，but the malignancy is not high. Overweight，obesity and“three high”are closely related to TN. Female，age and middle school teachers are risk factors for TN，so comprehensive attention should be paid to the above indicators to improve health management awareness.