Objective：To investigate the effects of venetoclax(ABT-199)combined with decitabine(DAC)and/or aclarubicin(ACM) on the proliferation and apoptosis of acute myeloid leukemia(AML)cell lines(HL60 and KG -1). Methods：HL60 and KG -1 were treated with different concentrations of ABT-199，DAC，ACM，ABT-199+DAC，ABT-199+ACM and ABT-199+ACM+DAC. The cell proliferation was determined by CCK-8 method. Compusyn software was used to analyze whether the drug combinations had synergistic effect. The apoptosis and cell cycle were detected by flow cytometry(FCM). Results：ABT-199，DAC and ACM inhibited the growth of HL60 and KG -1 respectively. The three ways of drug combinations significantly inhibited the cell proliferation of HL60 and KG -1， compared with ABT-199 alone，and all had synergistic effect. The combinations of ABT-199 significantly induced the apoptosis of HL60 and KG-1. The cell cycle was arrested at G2/S phase in HL60 and KG-1 cells treated by DAC，the combination of ABT-199 and DAC，or the combination of ABT-199，ACM and DAC. Conclusion：ABT-199 combinations significantly inhibited the proliferation of AML cell lines and had synergistic effect. ABT-199 combined with ACM and DAC promoted the apoptosis and arrested the cell cycle of AML cells.