Objective：To investigate the distribution characteristics of common human leukocyte antigen(HLA)haplotypes in Han population in Chinese Marrow Donor Program(CMDP)Jiangsu Branch from 7 prefecture-level cities in Jiangsu Province. Methods：In this study，11 889 volunteers from CMDP Jiangsu Branch were genotyped at HLA-A/B/DRB1/C/DQB1 loci using PCR-SBT method. HLA haplotypes frequencies of HLA-A-B-C，DRB1-DQB1，A-B-DRB1，A-B-DRB1-C-DQB1 were separately calculated using Arlequin 3.5 from 7 prefecture-level cities in Jiangsu Province，the common HLA haplotypes were statistically analyzed and compared with the published data of Han population in China. The clustering analysis between different populations was conducted by hierarchical clustering method with SPSS software. Results：In the HLA-A-B-C haplotypes，A*30∶01-B*13∶02-C*06∶02 and A*33∶03-B*58∶01- C*03∶02 were the top two common haplotypes in 7 prefecture -level cities of Jiangsu Province. The clustering analysis showed that Nanjing，Yangzhou，Changzhou and Zhenjiang were clustered，and Suqian，Huai’an and Xuzhou were clustered. The top two common haplotypes in HLA-DRB1-DQB1 haplotypes were DRB1*07∶01-DQB1*02∶02 DRB1*09∶01-DQB1*03∶03 in Suqian，Huai’an and Xuzhou，and DRB1*09∶01-DQB1*03∶03，DRB1*07∶01-DQB1*02∶02 were the top two common haplotypes in Changzhou，Yangzhou， Zhenjiang and Nanjing. The clustering analysis showed that 7 prefecture-level cities in Jiangsu Province were clustered into one group. Among the HLA-A-B-DRB1 haplotypes，A*30∶01-B*13∶02-DRB1*07∶01 was the most frequent haplotype in 7 prefecture-level cities of Jiangsu Province，and A*33∶03-B*58∶01-DRB1*03∶01 was the second frequent A-B-DRB1 haplotype in Nanjing，Yangzhou，Huai’ an and Changzhou. The clustering analysis showed that Nanjing，Yangzhou，Changzhou，Heilongjiang and Liaoning were clustered，and Huai’an，Suqian and Xuzhou were clustered. The most frequent HLA-A-B-DRB1-C-DQB1 haplotype was A*30∶01-B*13∶02-DRB1* 07∶01-C*06∶02-DQB1*02∶02 in 7 prefecture-level cities of Jiangsu Province，and the frequency of the haplotypes that containing A* 30∶01-B*13∶02-DRB1*07∶01 gradually decreased from north to south in Jiangsu Province. The clustering analysis showed that Changzhou，Xuzhou and Nanjing were clustered together，and then clustered with Zhenjiang. Huai’an and Suqian were clustered together，and then clustered with Xuzhou. Yueyang，Zhejiang and Guangdong were clustered together. Conclusion：On the whole，the three prefecture-level cities in Northern Jiangsu(Suqian，Huai’an and Xuzhou)are clustered together，and the other 4 cities are clustered together. The HLA haplotype polymorphism in Han population from 7 prefecture level cities of Jiangsu Province is more biased to the Northern China than the Southern China.