Objective：To investigate the effects of extracellular vesicles(EVs)from glioblastoma stem cells(GSC)on the proliferation，invasion and temozolomide(TMZ)- resistance of glioblastoma(GBM)in vitro and in vivo. Methods：The GSCs were derived from the primary GBM patient and the matched differentiated glioma cells(DGCs)were obtained through differentiation experiments. The EVs in culture medium of GSC and DGC were obtained by ultracentrifugation，respectively. Cell Counting Kit 8 (CCK8)assay，colony formation assay and transwell invasion assay were used to detect the effects of GSC - derived EVs on the proliferation，invasion and TMZ - resistance of U87 glioma cells. Intracranial xenografts murine experiment was used to validate the effects of GSC-derived EVs on the in vivo proliferation，invasion and TMZ-resistance of U87 cells. Results：GSC and the matched DGC were obtained through tumor dissociation and differentiation experiments，respectively. GSC and DGC derived EVs were obtained by ultracentrifugation methods. GSC-derived EVs significantly enhanced U87 cell proliferation，invasion and TMZ-resistance，compared to DGC-derived EVs. Consistently，in vivo experiments validated that GSC-derived EVs promoted tumor growth and reduced the survival time of mice. Conclusion：GSC-derived EVs enhance the proliferation，invasion and TMZ-resistance of glioma cells.