Objective：To investigate the expression and clinical significance of Casitas B-lineage lymphoma(Cbl)protein family in cervical squamous cell carcinoma(CSCC). Methods：Immunohistochemistry was used to detect the expression of c-Cbl，Cbl-b，and Cbl-c in 114 cases of CSCC. The correlations between c-Cbl，Cbl-b，and Cbl-c expression and clinicopathological characteristics，disease free survival(DFS)and overall survival(OS)were analyzed. Results：Of the 114 CSCC patients，87 had high c-Cbl expression and 27 had low c-Cbl expression；69 had high Cbl-b expression and 45 had low Cbl-b expression；55 had high Cbl-c expression and 59 had low Cbl-c expression. The histochemistry score(H - score)of c -Cbl in CSCC tissues and the adjacent normal tissues was 143.80 ± 8.76 and 95.13 ± 6.54，respectively. c-Cbl expression was increased in CSCC compared with the adjacent normal tissues(P ＜ 0.001). The H-score of Cbl-b in CSCC tissues and the adjacent normal tissues was 83.95 ± 5.28 and 93.54 ± 3.91，respectively. There was no significant difference in Cbl-b expression between CSCC tissues and the adjacent normal tissues(P=0.146). The H-score of Cbl-c in CSCC tissues and the adjacent normal tissues was 83.62 ± 6.34 and 36.54 ± 4.64，respectively. Cbl-c expression was increased in CSCC compared with the adjacent normal tissues(P ＜ 0.001). c-Cbl expression was higher in highly to moderately differentiated tumors and tumors with a maximum diameter less than 4 cm(both P ＜ 0.05)，but it was not related to the patient’s age，vascular invasion，depth of invasion， lymph node metastasis and FIGO stage(all P ＞0.05). Cbl - b expression was higher in patients with invasion depth ＞ 2/3 of fibromuscular layer(P=0.045)，but it was not related to patient’s age，vascular invasion，tumor differentiation，maximum tumor diameter，lymph node metastasis and FIGO stage(all P ＞ 0.05). Cbl-c expression was higher in poorly differentiated tumors(P=0.012)， but it was not related to patient’s age，vascular invasion，depth of invasion，maximum tumor diameter，lymph node metastasis and FIGO stage(all P ＞ 0.05). Kaplan - Meier survival analysis showed that patients with high c - Cbl expression had prolonged DFS and OS compared with those with low c-Cbl protein expression(both P ＜ 0.05). However，the expression levels of Cbl-b and Cbl-c were not related to DFS and OS(P ＞ 0.05). Cox univariate and multivariate analysis showed that c - Cbl expression was an independent prognostic factor for DFS(P=0.042). c -Cbl was a factor affecting OS，but not an independent prognostic factor for OS in CSCC(P= 0.096). Conclusion：Patients with high c -Cbl expression have prolonged DFS and OS compared with those with low c -Cbl protein expression. c-Cbl expression is an independent prognostic factor for DFS，but not an independent prognostic factor for OS in CSCC. The expression levels of Cbl-b and Cbl-c are not related to DFS and OS in CSCC. Detection of the expression status of c-Cbl may provide a reference for predicting the prognosis of CSCC patients.