Objective：To investigate the feasibility of secondary superovulation scheme in improving the superovulation effect and embryonic development potential of adult mice. Methods：Firstly，the difference of total egg number among the conventional double ovulation scheme，the natural ovulation and random superovulation experimental schemes was compared，and then the conventional double ovulation experimental scheme was further optimized on the basis of the preliminary study of the effect of exogenous pregnant mare serum gonadotropin(PMSG)on the development of naturally conceived embryos. Results：The average total number of eggs in the optimized double ovulation experimental group was 61.7，which was significantly different from the natural ovulation group，the random superovulation group and the conventional double ovulation group，and improved the superovulation effect of adult mice. Conclusion： The optimized double superovulation scheme provides a new and efficient technical method for obtaining a large number of mouse multi -cell embryos and fertilized eggs.