Objective：This study aims to explore the safety and feasibility of neoadjuvant chemotherapy combined with Da Vinci robotic surgery for advanced gastric cancer. Methods：The respective cross - sectional study was conducted. There were 151 patients retrospectively analyzed，who underwent radical gastrectomy after two cycles of neoadjuvant chemotherapy(SOX)in the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine from July 2018 to July 2022，and 120 cases were finally included according to the inclusion and exclusion criteria. According to mode of operation，all patients were divided into Da Vinci robotic radical gastrectomy group(robot group，60 cases)and laparoscopic radical gastrectomy group(laparoscopy group，60 cases). The general data， perioperative surgery related indexes and postoperative rehabilitation were compared between two groups. Results：There was no significant difference between the two groups in age，body mass index，tumor size，tumor location，surgical scope，digestive tract reconstruction mode，preoperative clinical stage(cTNM)，pathological stage(ypTNM)，cell differentiation degree，tumor regression grade，complete remission and partial remission(P ＞ 0.05). There were significant differences between the two groups in operation time，estimated intraoperative bleeding and abdominal incision length(P＜0.05). There were significant differences between the two groups in postoperative pain score，the time of first fluid diet，the time of first ventilation，the time of drainage tube extubation，the time of postoperative hospitalization and the cost of hospitalization(P ＜ 0.05). There were also significant differences in C-reactive protein， leukocyte count，neutrophil count，serum prealbumin，interleukin(IL)- 6 and procalcitonin between the two groups(P ＜ 0.05). However，there was no significant difference in the incidence rate of postoperative complications，readmission within 30 days after surgery，and secondary surgery within 30 days after surgery between the two groups(P ＞ 0.05). In the robot group，the estimated amount of surgical bleeding，the length of abdominal incision，the score of postoperative pain，the time of first liquid diet，the time of first ventilation，the time of extraction of abdominal drainage tube，and the time of postoperative hospitalization were better than those in the laparoscope group，but the operation time and hospitalization cost were significantly worse than those in the laparoscope group. Conclusion：For advanced gastric cancer，the strategy of preoperative neoadjuvant chemotherapy combined with Da Vinci robotic surgery is safe and feasible，and the incidence of postoperative complications is similar to laparoscopic surgery. So，Da Vinci robotic surgery system is a new candidate for advanced gastric cancer.