Objective：The aim of this study was to evaluate the associations between autonomic nervous function and constipation symptoms，psychological status and quality of life in function constipation(FC)patients. Methods：The characteristics，psychological status and heart rate variability(HRV)test of 102 FC patients and 42 healthy controls were evaluated. Then the FC patients were divided into three groups according to the result of HRV test，the autonomic tone balance normal group，the balance severely sympathetic tone group and the balance severely parasympathetic tone group. The results of self - rating anxiety and depression scale (SAS/SDS)，constipation scoring system(CSS)and patient assessment of constipation symptom(PAC - SYM)，patient assessment of constipation quality of life(PAC-QOL)were compared among three groups. Meanwhile，anorectal manometry and electrointestinogram parameters were assessed. We also analyzed the potential association of PAC - QOL with ANS function，mental status，constipation symptoms in all the constipation patients. Results：Compared with the healthy control group，the scores of SAS and SDS in FC patients increased significantly. HRV test results showed that the abnormal rates of autonomic nervous function were higher in FC patients than that in normal controls. Anorectal physiology and intestinal electric activity showed no significant difference among the FC patients with different HRV. Compared with patients in the autonomic tone balance normal group，patients in the balance severely sympathetic tone group had higher SAS/SDS scores，higher PAC - SYM score and lower PAC - QOL，especially for the dimension of“physical discomfort”and“worry/anxiety”of PAC -QOL. The balance severely parasympathetic tone group showed higher CSS score than the other two groups which maybe correlated with the prominence of depressed emotion. Multiple regression analysis showed that anxiety/ depression emotion and ANS function state could significantly affect the quality of life. Conclusion：FC patients with autonomic dysfunction are prone to anxiety or depression. The QOL of FC patients correlates strongly with ANS function and emotion，but not symptoms. Treatrnents such as autonomic adjustment，psychological interventions and central nervous system drugs may have clinical significance for FC patients.