Objective：To explore the role of mTOR signaling pathway in CD4 ⁺ regulatory T cell(Treg)glucose metabolism in ovarian cancer patients. Methods：Flow cytometry was used to detect the percentage of mTOR⁺ CD4 ⁺ Treg in peripheral blood with ovarian cancer(OC)patients，benign ovarian tumor(BOT)patients and healthy controls(HC). We established a coculture system of human CD4⁺ Treg with ovarian cancer cell SKOV3，and detected the percentage of mTOR⁺ CD4⁺ Treg before and after co-culture. After mTOR signal was inhibited by rapamycin，the glucose uptake and glycolysis levels of CD4⁺ Treg were detected by colorimetry，and the expression levels of genes and proteins related to glucose metabolism in CD4 ⁺ Treg were detected by real-time quantitative PCR and Western blot. Results：The percentage of mTOR⁺ CD4⁺ Treg in peripheral blood of ovarian cancer patients was significantly higher than that of healthy controls[(77.4±8.12)% vs(. 64.19±9.7)%，P ＜ 0.01]. After co-culture with SKOV3，the percentage of mTOR⁺ CD4 ⁺ Treg were elevated(P ＜ 0.05)，and the levels of mTOR signaling pathway related genes and proteins also increased. After inhibition of mTOR signal of CD4 ⁺ Treg in SKOV3 growth environment，the glucose uptake(193.49 ± 13.28 vs. 174.05 ± 14.58，P ＜ 0.01)and glycolysis level(21.97±0.87 vs. 4.85±1.54，P ＜ 0.001)were decreased significantly. Glucose metabolism - related genes and proteins were also significantly reduced. Conclusion：mTOR and its downstream signaling pathway are significantly activated in peripheral blood CD4 ⁺ Treg of ovarian cancer patients，and mTOR signaling pathway can affect glucose metabolism of CD4 ⁺ Treg by regulating glucose metabolist-related genes and protein levels.