Objective：Based on the glycosylation related gene set，the TCGA-GTEx joint public database was used to identify genes significantly related to the occurrence and poor prognosis of pancreatic cancer，and the tissue samples of our center and in vitro experiments were used for verification. Methods：The glycosylation gene B4GALT5 related to the risk and poor prognosis of pancreatic cancer was screened through the public database，and was verified using the tissue samples of our center with clinical and prognostic information. We constructed pancreatic cancer cell lines with B4GALT5 interference and overexpression，and observed the effects of B4GALT5 on the malignant biological behavior of pancreatic cancer cells in vitro through CCK -8，wound healing，transwell invasion experiments. Results：The expression of B4GALT5 in pancreatic cancer tissue was significantly higher than that in normal pancreatic tissue. The increased expression of B4GALT5 was positively correlated with the risk and poor prognosis of pancreatic cancer，and negatively correlated with the infiltration of CD4 ⁺ T cells and NK T cells. B4GALT5 played a role in exacerbating the proliferation， movement and invasion of pancreatic cancer cells in vitro. Conclusion：The glycosylation gene B4GALT5 can promote the malignant progression of pancreatic cancer，and may be used as a new biomarker to indicate the risk and poor prognosis of pancreatic cancer.