Objective：The current study aims to synthesize hypochloric acid（HClO）fluorescent probes targeting myeloperoxidase （MPO）and evaluate its HClO tracing activity. Methods：Si-rhodamine was used as the fluorophore to synthesize SiRho-GABA-KYC. The UV spectrum，fluorescence spectrum，and pH stability and selectivity were detected with UV spectrophotometer and fluorescence spectrophotometer. Cytotoxicity was detected with CCK - 8 method，and cell fluorescence imaging was performed by confocal microscopy. Results：SiRho -GABA -KYC was synthesized，with a high response to HClO，a good selectivity，and a low cytotoxicity， which can trace changes in HClO concentration in HeLa cells. Conclusion：The probe SiRho-GABA-KYC can be used to detect HClO concentration，which in turn reflects MPO catalytic activity.