Objective：To construct lecithin - cholesterolacyl transferase（LCAT）knock -in mice by CRISPR/Cas9 - mediated gene editing and to obtain liver-specific overexpression of Lcat mice by mating with liver-specific Cre-expressing transgenic mice. Providing an animal model for the study of the mechanism of the Lcat gene in the development of liver-related metabolic diseases. Methods：Lcat knock -in mice were constructed by CRISPR/Cas9 technology；Liver - specific Cre- expressing transgenic mice were mated with Lcat knock -in mice to obtain liver -specific overexpressing Lcat mice；Genotyping mice by PCR；Quantitative real -time PCR（qPCR）and Western blot（WB）techniques were used to verify the expression of Lcat gene in C57BL/6 mice. Results：The PCR results showed that the liver-specific overexpression of Lcat gene in mice was successfully constructed；the qPCR results showed that the Lcat gene was specifically highly expressed in the liver，and the liver of knock -in mice showed higher Lcat expression；the WB results showed that LCAT protein was more highly expressed in the liver of liver-specific Lcat knock-in mice. Conclusion：Liver-specific overexpression of Lcat gene mice were successfully constructed，providing a platform for exploring the function of the Lcat gene at animal level in liver-related metabolic diseases and the associated pathogenesis.