Objective：The current study aims to investigate the effect and mechanism of signal transducer and activator of transcription 1（STAT1）on cancer-related gene Gas6. Methods：The database was used to predict the results of the gene cluster analysis of breast cancer，and to predict the correlation between Gas6 and STAT1 in multiple cancers. The binding of STAT1 to Gas6 promoter was tested by chromatin immunoprecipitation（ChIP）assays. The binding sites of STAT1 on Gas6 promoter were predicted by bioinformatics analysis and identified by mutation plasmid construction，and the effects of binding site mutation on activity of Gas6 promoter was detected by dual luciferase reporter assay. The effects of knockdown and overexpression of STAT1 on Gas6 mRNA and protein expression were measured by qRT-PCR and Western blot，respectively. Results：Gas6 was a highly expressed gene in breast cancer. Gas6 was positively associated with STAT1 in multiple cancers. The Gas6 promoter contained binding sites of STAT1. STAT1 positively regulateed Gas6 at transcriptional levels. Conclusion：The cancer related gene Gas6 is a direct target gene for transcription factor STAT1.