Objective：To analyze the urinary tract infection situation，pathogens，and drug resistance characteristics in male urethral stricture patients. Methods：Male patients with urethral stricture admitted to our hospital from January 2021 to August 2022 were enrolled. Clinical data and laboratory test results were collected to analyze the urinary tract infection，pathogen distribution，and drug resistance situation in the patients. Results：A total of 187 male urethral stricture patients were included，with132 cases（70.6%） experiencing urinary tract infections. Microbiological testing revealed positive urine cultures in 106 cases（56.7%）. A total of 111 strains of isolated pathogens were cultured，including 42 strains of Gram-positive bacteria，accounting for 37.8% ，mainly Staphylococcus epidermidis and Enterococcus faecalis，with resistance rate to levofloxacin exceeding 70% and sensitivity rates to vancomycin，tigecycline and nitrofurantoin all at 100%；63 strains of Gram-negative bacteria，accounting for 56.8%，mainly Escherichia coli and Klebsiella pneumoniae，with resistance rate to cefotetan，tobramycin，meropenem，piperacillin/tazobactam，imipenem，and amikacin all below 30%；and 6 strains of fungi，accounting for 5.4%，mainly Candida glabrata. The proportion of patients aged 40-65 years and those with suprapubic catheter in the urinary tract infection group was significantly higher than that in the non-infection group （P < 0.05）.Conclusion：The incidence of urinary tract infection is high in male urethral stricture patients，with the main pathogens being E.coli and S.epidermidis. The drug resistance of the pathogens is relatively strong. Antimicrobial agents should be used rationally according to drug sensitivity before surgery.