Objective：This study aimed to investigate the physicochemical properties of a three-dimensional（3D）composite scaffold loaded with curculigoside（CUR）and assess its potential impact on promoting angiogenesis and osteogenic induction in human umbilical vein endothelial cells（HUVEC）and mouse bone marrow mesenchymal stem cells（BMSC）. Methods：Polycaprolactone microspheres loaded with Curculigoside（CUR-PM）were prepared using an emulsion/solvent evaporation technique. We successfully engineered a 3D composite scaffold comprising hydroxyapatite（HA），gelatin（GEL），and sodium alginate（SA）with the assistance of 3D bioprinting technology，denoted as HGS. Furthermore，we established a polycaprolactone-based microsphere scaffold，referred to as HGSC，for the purpose of CUR loading. The scaffold was characterized by scanning electron microscopy，infrared spectroscopy， rheology，mechanical properties，drug release，and degradation experiments. The biocompatibility of the scaffold was verified using CCK-8 and EdU fluorescence staining experiments. The potential of the HGSC scaffold to promote cell vascularization and bone regulation was assessed through HUVEC tube formation experiments and alkaline phosphatase（ALP）staining. Results：The HGSC exhibited a uniform grid-like structure with appropriate mechanical properties and degradation compared to HGS. The CCK-8 and EdU fluorescence staining demonstrated excellent biocompatibility of the HGSC scaffold. The outcomes of HUVEC tube formation experiments and BMSC ALP staining provided evidence that the HGSC scaffold exhibited the potential in promoting angiogenesis and osteogenesis. Conclusion：The HGSC scaffold has shown excellent biocompatibility and has a clear promoting effect on the osteogenic potential of BMSC and angiogenesis of HUVEC. This study offers a promising therapeutic strategy for bone defect repair.