Objective：To construct a Bandavirus davieense virus（DBV）vaccine based on the strategy of mRNA vaccine，immunize BALB/c mice，and evaluate its immunologic characteristics. Methods：The coding sequence of DBV glycoprotein Gn was optimized and synthesized，then inserted into the pGEM -3Zf（+）plasmid. The linearization plasmids were enzyme digested by BamH Ⅰ，cap and polyA tailing were added with polymerase to complete in vitro transcription. Western blotting verified the protein expression by transient transfection of the mRNA into eukaryotic 293FT cells. The mRNA was delivered by lipid nanoparticles，and immunized BALB/c mice by intramuscular injection of low（2 μg/mouse），medium（5 μg/mouse）and high（20 μg/mouse）dose once every two weeks. The antibody titer in mice serum was detected by ELISA，and the ability of antibody to neutralize DBV in vitro was detected at the cellular level by virus neutralization assay. Results：The prepared DBV mRNA vaccine could be transcribed and expressed in vitro. All three immunization doses induced high levels of specific antibodies in mice，with the high-dose immunization group inducing stable antibody expression for over 10 weeks. The virus neutralization assay showed that immune sera could bind to the virus and block virus infection of cells. Conclusion：A DBV mRNA vaccine capable of inducing specific neutralizing antibodies in mice is obtained，laying the foundation for research on the prevention of DBV infection.