Objective：To investigate the diagnostic value of balloon-assisted small enteroscopy and other examination methods for Meckel’s diverticulum of the small intestine. Methods：A retrospective analysis was conducted on 87 patients diagnosed with Meckel’s diverticulum at the First Affiliated Hospital of Nanjing Medical University from January 2010 to December 2023. Data regarding demographics，clinical presentations，diagnostic methods，treatments，and pathological findings were collected. Clinical characteristics were summerized，and the diagnostic efficacy of balloon-assisted small enteroscopy was compared with other examination methods for detecting Meckel’s diverticulum. Results：A total of 87 patients with Meckel’s diverticulum were included，with 55 patients had complications such as gastrointestinal bleeding，intestinal obstruction，diverticulitis，and intestinal perforation. Compared to patients without complications，those with complications had a younger median age（P=0.003），longer diverticulum diameter[（5.2 ± 2.6）cm vs. （3.3 ± 1.4）cm，P=0.001]，wider opening[（2.4 ± 1.1）cm vs.（ 1.6 ± 0.7）cm，P=0.002]，and higher surgery rate[（89.1% vs. 56.3%，P ＜ 0.001]. The diagnostic sensitivity of different examination methods for Meckel’s diverticulum were as follows：balloon-assisted small enteroscopy 94.4%，nuclide imaging（ectopic gastric mucosal imaging）58.3%，capsule endoscopy 30.0%，small intestine CT 9.4%， abdominal CT 8.2%，and digital subtraction angiography 0%. Conclusion：Young patients with longer diverticulum diameter and wider opening of small intestine Meckel’s diverticulumin are more prone to complications such as diverticulitis，perforation，bleeding，and obstruction. Balloon-assisted small enteroscopy has the highest preoperative diagnostic value for Meckel’s diverticulum and should be the preferred examination method for patients suspected of having small intestine Meckel’s diverticulum.