Objective：To assess the value of sampling perfection with application optimized contrast using different flip angle evolution（SPACE）sequence combined with compressed sensing（CS）technique in the diagnosis of acromioclavicular joint injury. Methods：Total 34 patients with a history of shoulder trauma and suspected acromioclavicular joint injury in the First Affiliated Hospital of Nanjing Medical University were prospectively included form May，2023 to February，2024. Conventional 2D magnetic resonance sequence and 3D CS-SPACE sequence scanning based on CS were performed on the patients. Signal intensity and standard deviation of long head tendon of biceps and bone marrow cavity of humerus were measured on two sets of images，and SNR and CNR were calculated. Three doctors assessed the injuries of the acromioclavicular joint through two sets of images and gave their diagnostic confidence rating. The SNR，CNR of bone marrow cavity and biceps long tendon，and diagnostic confidence ratings were compared between the two groups. The diagnostic consistency of the three doctors in conventional 2D images and 3D CS - SPACE images was analyzed respectively，and the diagnostic consistency between the two sets of images was evaluated. Results：In the objective evaluation of image quality，SNR and CNR of 3D CS-SPACE image were obviously better than that of conventional 2D image. For the two groups of images，two doctors’ratings of 3D CS-SPACE images were significantly higher than those of conventional 2D images，and one doctor’s ratings had no significant difference. The three doctors demonstrated a strong agreement in the evaluation of acromioclavicular joint injury on both conventional 2D images and 3D CS-SPACE images（κ＞0.6），and there was a high consistency in the evaluation of acromioclavicular joint injury between the two groups of images（κ ＞0.6）. Conclusion：For the diagnosis of acromioclavicular joint injury，3D CS-SPACE sequence images has high consistency with conventional 2D images，and 3D CS-SPACE sequence can shorten the scanning time and obtain better image quality.