Objective：This study aimed to investigate the therapeutic effect of burdock polysaccharide on Alzheimer’s disease（AD） using Caenorhabditis elegans（C. elegans）pathology model. Methods：Burdock polysaccharide made in our lab was used at varying concentrations（62.5，125.0，and 250.0 μg/mL）on transgenic C. elegans AD models（CL4176，CL2355，and CL2006 strains）to study the effects on nematode amyloid beta（Aβ）deposition，chemotaxis，5-hydroxytryptamine sensitivity，paralysis，growth and development， locomotor activity，feeding ability. Oxidative stress and cholinergic neuron-related indexes were measured，and the expression levels of related genes in nematodes were examined by RT -PCR. Results：Burdock polysaccharide exhibited multiple biological activities， thus reducing the level and deposition of Aβ protein，reducing 5-hydroxytryptamine sensitivity，strengthening nematode chemotaxis and learning memory，delaying paralysis，promoting development，increasing swing and pharyngeal vibration frequency，increasing antioxidant enzyme activity and reducing acetylcholinesterase（AChE）activity. Meanwhile，it reduced the expression of age-1 and akt-1 genes，enhanced the expression of daf-16 gene，promoted the expression of skn-1，gst-4，sod-1 and sod-2，exert antioxidant effects，and alleviated AD symptoms. Concurrently，it attenuated AD pathology by inhibiting ace -1/ace -2 expression，and reducing AChE activity. Conclusion：Burdock polysaccharide may alleviate and improve AD-related pathological processes by regulating the age-1/akt-1/daf-16 signaling pathway to reduce oxidative stress levels while inhibiting AChE activity.