Objective：To screen differentially expressed microRNAs in serum of normal pregnancy and preeclampsia（PE）patients， and analyze them in conjunction with hematological and biochemical indicators，aiming to provide predictive value for early PE diagnosis. Methods：MicroRNA dataset related to PE was downloade from the GEO database，and differentially expressed microRNAs were screened using the DESeq2[1.36.0]package，followed by experimental validation in serum samples. Serum samples were collected from 37 PE patients who underwent antenatal examinations and delivered at Nanjing Maternity and Child Health Care Hospital from January 2024 to July 2024，as well as 33 age-and gestational week-matched normal pregnant controls. Total serum microRNAs were extracted，and the expression levels were detected by quantitative fluorescence PCR. At the same time，hematological parameters and biochemical test data were collected. Independent sample t-test was used to analyze the differences in test results，and chi-square test was used to compare the differences in complications between the two groups. In addition，the value of the screened microRNAs and their combination with blood biochemical indicators in predicting and diagnosing preeclampsia was evaluated by using the receiver operating characteristic（ROC）curve. Results：Two microRNA datasets-GSE234611（early-onset PE vs. normal pregnancies）and GSE118578（primary hypertension vs. normal individuals）-were downloaded from GEO. Using DESeq2[1.36.0]，four overlapping differentially expressed microRNAs were identified（hsa-miR-106b-5p，hsa-miR-24-3p，hsa-miR-451a，and hsa-miR-92b- 3p）. Subsequent serum validation revealed significantly elevated expression of hsa-miR-451a and hsa-miR-106b-5p in the PE group （P ＜ 0.05），while hsa-miR-24-3p and hsa-miR-92b-3p showed no significant differences（P ＞ 0.05）. Hematological and biochemical indicators analyses indicated that the PE group had significantly lower platelet counts and neutrophil-to-lymphocyte ratios but higher lymphocyte counts，mean platelet volumes，uric acid，and homocysteine levels compared to controls（P ＜ 0.05）. ROC curve analysis showed that the area under the curve（AUC）values for hsa-miR-451a，hsa-miR-106b-5p，uric acid，and homocysteine in diagnosing PE were 0.827，0.931，0.801，and 0.704，respectively. The combined AUC of hsa-miR-451a，uric acid，and homocysteine was 0.908，with a sensitivity of 72.22% and a specificity of 94.12%，while the combined AUC of hsa -miR -106b -5p，uric acid，and homocysteine was 0.941，with a sensitivity of 94.44% and a specificity of 88.23%. The incidence of adverse pregnancy complications in the PE group was significantly higher than that in the control group（P ＜ 0.05）. Conclusion：Combined detection of serum hsa-miR-106b-5p，uric acid， and homocysteine has predictive and diagnostic value for PE.