Objective: Nanomedicine is promising in conquering the tumor, while the traditional nano-vector is limited by poor stability, short blood circulation, and high immunogenicity. Hence, developing a novel nano-system with sterling stability and non-specific protein resistance for drug delivery is urgent. Methods:To overcome these challenges, we design a biodegradable carboxybetaine (CB)-modified polycarbonate (PAC(TCB)) synthesized by ring-opening polymerization and Michael addition reaction, which can form the micelles of around 160 nm via solvent displacement method. Results:We have demonstrated that the developed zwitterionic micelle possesses the superior characteristics of efficient cell internalization, excellent biocompatibility, low immunogenicity, and prolonged blood circulation. Moreover, our devised micelles will not produce antibody effects after repeated injections. Conclusion:Accordingly, the biodegradable carboxybetaine (CB)-modified polycarbonate displays colossal potential as the nano-vector in drug delivery.