Objective: To investigate the effect of tracheal tube cuff pressure control on intraoperative hemodynamics and postoperative sore throat during laparoscopic colorectal surgery. Methods: Ninety-four patients who underwent laparoscopic radical surgery for colorectal and rectal cancer were selected were randomly divided into Group A (tracheal tube cuff pressure control group, n = 48) and Group B (finger sensation method group, n = 46). The pressure of the tracheal tube cuff in Group A was controlled at 25-30 cmH₂O, while the pressure in Group B only be monitored. HR and MAP were continuously monitored and recorded at seven time points including before induction of anesthesia (T0), after intubation (T1), before establishment of pneumoperitoneum (T2), after establishment of pneumoperitoneum (T3), after Trendelenburg position (T4), before tracheal extubation (T5), after tracheal extubation (T6). Tracheal tube cuff pressure and airway pressures at the time points of T1, T2, T3, T4 and T5 were measured and recorded. The incidence and severity of sore throat and hoarseness were investigated in patients at 2 h, 12 h and 24 h after surgery. Results: The cuff pressure in Group B was significantly higher than that in Group A at T1, T2, T3, T4, and T5 (P < 0.05). The airway pressure in both groups significantly increased at T3 and further increased at T4 (P < 0.05), but there was no significant difference between the two groups. Compared with those in group A, HR and MAP were higher in group B at T1, T2, T3, T4 and T5 time points (P < 0.05). The incidence of postoperative sore throat at 2 h and 12 h was lower in group A than that in group B (P < 0.05). There was no statistical difference in the incidence and severity of postoperative hoarseness at 2 h, 12 h and 24 h in the two groups (P > 0.05). Conclusion: In patients undergoing laparoscopic colorectal surgery, tracheal tube cuff pressure control can reduce the incidence of postoperative sore throat and maintain the relative stability of intraoperative hemodynamics.