Objective: To establish a microbiological qualitative detection method for three antibiotics (chloramphenicol, ofloxacin, and cefixime) in cosmetics. Method: Prepare a cosmetic standard solution containing antibiotics to simulate the background matrix of actual cosmetic samples. Modified Letheen Broth (MLEB) was used to neutralize preservatives in the samples. The volume ratio of the sample, MLEB and bacterial solution（concentration is approximately 1×10^11 cfu/mL） is 1:9:2. After culturing at 37℃ for 45 and 90 minutes, the bacterial recovery rate is analyzed and compared with the standard recovery rate in order to make qualitative judgments. Results: The detection limit of this method for chloramphenicol, ofloxacin and cefixime are 5 μg/g, 1 μg/g and 1 μg/g, respectively. Conclusion: This method is convenient, cheap, accurate and suitble for the quick detection of 3 antibiotics in cosmetics.