Objective：To analyze the genetic characteristics of chromosomes of sperm donor volunteers in our sperm bank for more than ten years, and to explore the role of sperm bank chromosomal karyotypes analysis and screening in avoiding adverse pregnancy outcomes after sperm donor treatment. Methods：The chromosomalkaryotypes examination results of 3591 sperm donors who underwent sperm screening at the Jiangsu Human Sperm Bank from January 2012 to December 2023 were retrospectively analyzed to classify the distribution and types of chromosomal karyotypes abnormalities. Results：Among the 3591 sperm donors, there were 62 cases of karyotype abnormalities, accounting for 1.73% of all volunteers, of which the most common were pericentric inversions (30/62), followed by secondary constriction region enlargement (13/62), and then short-arm enlargement (7/62) andtranslocation (6/62). In addition to this, there were 3 cases of satelliteenlargement, 1 case of double enlargement, 1 case of enlarged centromere and 1 case of markerchromosome. Conclusion:In view of the known or potential adverse effects of karyotype abnormalities on pregnancy outcomes and offspring health, sperm banks are required to conduct rigorous cytogenetic screening of sperm donor volunteers to circumvent the risk of failure of subsequent sperm donor treatments and to fulfil the societal role of assisted conception and eugenics.