Objective To establish a VSMC cultivating system which is similar close to in vivo condition. Methods Cells derived from rabbit hyperplastic intima after arterial intima injury were cultured. Results These cells, identified by transmission electron microscope displayed both ultrastructural features of synthetic SMC and characteristic immunocytochemical staining for smooth muscle myosin. They were arranged in the form of “peak” and “valley”. Conclusion Cultured synthetic SMC is better than SMC cultured from the medium.