To investigate the neuroprotective effect and mechanism of TMB 8[8- (N, N-diethy lamino)- n -octyl- 3,4,5- trimethoxybenzoate]. Methods The oxygen-glucose deprivation model of cultured rat neu ron was used to observe the effects of TMB-8 on neuron morphology, permeability and contents of MDA, GSH, TSOD and GSH px in neurons. Results The damaged neurons induced by oxygen glucose deprivation for 1 h and after serum-free culture for 24 h could liberate much LDH; the contents of GSH and the activity of T SOD in neurons were significantly decreased; TMB-8 could concentration dependently reduce the efflux of LDH from the damaged neuron and increase the content of GSH in neuron, the activity of T-SOD in these cells was also increased. Conclusion the neuroprotective role of TMB-8 may be related to the increasing of GSH and T SOD in the damaged neuron.