Objective:The aim of the study was to identify the single nucleotide polymorphisms (SNPs) on type 2-diabetes susceptibility genes located on chromosome 9 D9s171. Methods: Putative type 2-diabetes susceptibility genes were selected based on their known potential involvement in insulin signaling pathway. Sequences of proximal promoter, coding regions (including sequence near splicing site) of each of 4 susceptibility genes were amplified by polymerase chain reaction (PCR). The PCR was carved out using DNA samples from 9 normal controls and patients of 15 type 2-diabetic pedigrees, which had high NPL Scores on D9sl71. PCR products were subjected to the direct bi-directional sequencing, and the sequencing data were analyzed with software Polyphred. SNPs in these genes among type 2 diabetics were detected. Results: A total of 14 SNPs were identified for 4 susceptibility genes; there were 11 high frequency SNPs and 3 low frequency SNPs. Conclusion: SNPs were identified in 4 candidate genes from patients of type 2 diabetes in East China, and these results should be confirmed or rejected in large sample genotyping study and association study in the future.