Objective： To investigate the relationship between reflux of acid／bilirubin and reflux， dyspepsia and symptoms from non-gastrointestinal tract， and to study the role of 24 h esophageal pH and bilitec monitoring in evaluating the new method of esophageal operation. Methods: Using the method of 24 h esophageal pH and bilitec monitoring， the acid and bilirubin in esophagus from 68 patients with typical reflux， 50 patients with dyspepsia； 20 patients with symptoms from non-gastrointestinal tract， and 32 patients undergoing esophageal operation were measured. Results：Of 68 patients with typical reflux， 65 presented with reflux episodes， with an incidence of 95.6%. Of 50 patients with dyspepsia symptoms，the incidence of reflux episodes was 72%. In patients with typical reflux， 51.4% presented with mixture reflux of acid and duodenal liquid. The incidence of reflux incidence in patients with new method of esophageal operation（preserving cardia and utilizing colon instead of esophagus） was lower， compared with the routine method（29.4% vs 66.7%，P < 0.05）. Conclusion： 24 h esophageal pH and bilitec monitoring provide an objective and effective method not only to diagnosis gastroesophageal reflux and duodenal-gastroesophageal reflux but also to evaluate the curative effect of the new operation method.