Development of abdominal transfered colon carcinoma rats model and the concentrations of hydroxyproline in colon stoma tissue after tumorectomy
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    Abstract:

    Objective:To develop a new kind of abdominal transfered colon carcinoma model in rats, and to evaluate the healing of stoma by measure the concentrations of hydroxyproline in tissue of colon stoma. Methods:Colon Carcinoma cell SW480 was inoculated subcutaneously into athymic mice to develop an implantation tumor,Subsequently the tumor lumps were implanted to the SD rats’ colon mucosa to setup the model. The tumor tissues were cut and the colons were anastomosed. 6 rats which were not implanted tumors but colons been cut and anastomosed were included into control group. Then measure the tensile strength and concentrations of hydroxyproline of colon stoma tissue with HPLC. Results:60% rats were success to establish abdominal transfered Colon Carcinoma models. The tensile strength and the concentrations of hydroxyproline in colon stoma tissue were both lower in experiment group than in control group(P<0.05.) Conclusion:It’s a reliable method to build tumor model and tumor significantly delays the healing of stoma.

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王国柱,吴文溪,张玉华.大鼠结肠癌腹腔转移模型的建立和肿瘤切除术后结肠吻合口羟脯氨酸含量的测定[J].南京医科大学学报(自然科学版英文版),2006,(12):1255-.

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  • Received:July 15,2006
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