Objective：To construct bicistronic DNA vaccines containing extra-cellular fragment of Flt3 ligand（FL） genes and early secreted antigen（ESAT-6） of Mycobacterium tuberculosis and to express them in glomerular mesangial cells（GMC）. Methods：FL and ESAT-6 genes were cloned into pIRES, a bicistronic vector, using polymerase chain reaction（PCR）. After being screened and identified, the vector was transfected into GMC cells in order to examine FL and ESAT-6 and their expression levels. Results：The recombinant vector was confirmed by sequencing and the expression of FL and ESAT-6 genes in GMC cells were detected by western blot. Conclusion：The recombinant pIRES-FL-ESAT6 bicistronic vector could be successfully constructed and expressed in vitro.