Objective：To establish GFPu-1 clonal cells reflecting the function of the ubiquitin-proteasome system. Methods：GFPu plasmid was transfected into human embryonic kidney（HEK） 293 cells. GFPu-1 cells with stable expression of GFPu were selected by G418. After proteasome inhibitor lactacystin was added into GFPu-1 cells, the distribution and expression of green fluorescent protein（GFP） were studied by confocal microscopy and Western blot. Results：GFPu was distributed diffusely in the nuclear and cytoplasmic compartment of GFPu-1 cells with lactacystin. Compared with the control cells with DSMO, the fluorescence of GFPu significantly increased in GFPu-1 cells with lactacystin. Accummuation of GFPu rose about 2.5 folds（P < 0.05） and β-catenin expression rose about 1.2 fold（P < 0.05）. Conclusion：GFPu-1 clonal cells may be a sensitive indicator for the function of the ubiquitin-proteasome system.