Objective：To investigate the role of extracellular signal-regulated kinases1／2（ERK1／2） in inschemia-reperfusion injuty of spinal cord. Methods：The morphology of spinal cord by hematoxylin and eosin（HE） stain and electron microscope in different ischemia／reperfusion time were observed. The activation of ERK1／2（pERK1／2） was detected by western blot and the localization of pERK1／2 was analyzed by immunohistochemistry. Results：ERK1／2 was activated in spinal cord occluded for 30 min and reperfused for 2 to 24 h. No pERK1／2 was found in neurocyte in the control group by immunohistochemistry. PERK1／2 was stained both in the cytoplasm and nucleus after 30 min of occlusion and 2 h of reperfusion, and stained further after 8 to 24 h of reperfasion. Conclusion：ERK1／2 was activated in the reperfusion of ischemic spinal cord and the stagnation of pERK1／2 in the cytoplasm during reperfusion of ischemic spinal cord may lead to neurocyte apoptosis.