Objective：To study the genetic polymorphisms of GSTM1，GSTT1 and GSTP1 and the susceptibility of rectal cancer. Methods：The study was conducted with 210 cases of rectal cancer and 439 cases of normal controls. The epidemiological data were collected, and the genotypes of the GSTM1 and GSTT1 were detected by multiplex PCR. Polymorphism of GSTP1（A→G） was detected using PCR-based restriction fragment length polymorphisms（PCR-RFL P）. Results：No significant difference was found in the frequencies of the deletion genotypes among the patients and controls No significant difference was found in the frequencies of GSTP1 A／A, A／G and G／G genotypes among the patients and controls. Creased risk of rectal cancer was not significanthy associated with GSTP1 G／G genotypes（adjusted for age, sex and status of the smoking and alcohol drinking（OR = 1.11, 95% CI：0.77-1.60） compared with those with GSTP1 A／A genotypes. Conclusion：The polymorphisms of GSTM1，GSTT1 and GSTP1 may not be associated with the susceptibility of rectal cancer.