Objective：To construct a plasmid pET32a-EC2A for the expression of recombinant extracellular gene of LMP2A of Epstein-Barrin virus（EBV） in escherichia coli（E.coli） and utilize EC2A protein in serological detection for patients with nasopharyngeal carcinoma（NPC）. Methods：The plasmid pEC2A containing the recombinant extracellular gene of LMP2A of EBV was digested with BamHⅠ and EcoRⅠ and cloned into the expression vector pET32a. The constructed vector which had been identified by PCR, enzyme digestion and nucleotide sequences analysis was transformed into BL21. After induced with IPTG, the recombinant protein was purified with Ni+ affinity chromatography, at the same time, its antigenic activity was identified by Western blot. The purified protein was used in serological detection for patients with NPC which is associated with EBV. Results：The recombinant plasmid was constructed correctly. SDS-PAGE and Western blot analysis showed that the recombinant protein was about 40 ku. The purified protein could be used as an antigen to detect specific antibodies in the sera of patients with NPC. Conclusion：The recombinant extracellular gene of LMP2A of EBV could be expressed with high performance and the purified protein may be used in serological detection of antibodies for patients with NPC.