Objective：To observe the effect of human recombinant hepatocyte growth factor（HGF） on the expression of vascular endothelial growth factor（VEGF） in colorectal carcinoma（CRC） cells in vitro. Methods：Human CRC cell lines SW480, LOVO were cultured with different concentrations of HGF. The culture supernatants were collected to detect the production of VEGF by ELISA assay. The effect of HGF on phosphorylation of its receptor c-Met was detected by Western blot assay. The expression of VEGF mRNA stimulated by HGF was investigated by reverse transcription PCR（RT-PCR）. Results：HGF increased the expression of VEGF （mRNA 4-5-fold and protein 3-10-fold） in CRC cell lines; HGF induced the phosphorylation of its receptor c-Met in a dose-dependent manner. Conclusion：In vitro, HGF can up-regulate the expression of VEGF in CRC cells, which correlates with the phosphorylation of its c-Met induced by HGF. Inhibitor of phosphorylation-c-Met, HA, can inhibit the expression of VEGF in CRC cells induced by HGF.