Objective：To investigate the effects of 4’-methery-scutellarein（4-MS） on proliferation and intracellular calcium concentration in human choriocarcinoma JAR cell line. Methods：MTT assay, flow cytometry and laser scanning confocal microscopy were used to detect the effects of 4-MS on JAR cells. Results：4-MS inhibited the growth of JAR cells. Annexin Ⅴ-FITC +／PI- cells and intracellular calcium concentration were gradually increased with the increase of the dose and action time of 4-MS. There were significant differences compared with the control group. Conclusion：4-MS can inhibit the proliferation and induce apoptosis of human choriocarcinoma JAR cell line. Its mechanism is related to the increase of intracellular calcium concentration.