Objective：To study ureaplasma urealyticum（UU） and mycoplasma hominis（MH） infection in the urogenital duct and their drug-resistance. Methods：Mycoplasma culture was carried out with the samples of 682 cases and the drug-resistance was detected at the same time. Results：359 samples from 682 suspected cases cultured were identified to present with positive mycoplasma（52.64%）. Among them , 298 samples were infected by UU（43.7%）, 8 samples by MH（1.2%）, and 53 samples by both UU and MH（7.8%）. The detectable rate of UU was far higher than that of MH. Mycoplasma infection was chiefly found in middle-aged people ranging from 21 to 40 years old（86.63%）. The positive rate of the female was much higher than that of the male（P < 0.05）. The results of drug-resistance showed that mycoplasma was much sensitive to josamycin, pristinmycin, doxycycline, tetracycline, mid-sensitive to azithromycin and erythromycin, and resistant to ciprofloxacin,ofloxacin and clarithromycin. Conclusion：The urogenital mycoplasma infection results mainly from UU, especially in young and mid-aged people. Compared with simple UU or MH infection, mixed infection with UU and MH has significantly greater resistance to a wider variety of drugs. Josamycin, pristinmycin, doxycycline, and tetracycline are the primary choices for urogenital mycoplasma infection.