Objective:To investigate toxicity of deoxypodophyllotoxin(DOP) against 3rd instar larvae of Pieris rapae L. and its effects on activities of AChE and ATPase, and to study its insecticidal mechanism. Methods:The toxicity of DOP was measured with leaf-dipping method. The activites of enzymes were determined with AChE and ATPase assay kit. Result:① DOP exhibited toxicity against 3rd instar larvae of P. rapae L. This toxicity is concentration-time-dependent with LC50=151.87 mg/L(at 48 h)>LC50=39.99 mg/L (at 72 h)> LC50=10.60 mg/L(at 96 h).② DOP did not affect the activity of AChE. ③ DOP profoundly inhibited the activity of ATPase at high concentration(125 mg/L). DOP at middle concentration(5,25 mg/L) showed activative effect at 24 h,then recovered at 48 h, and maintained normal(5 mg/L) or exhibited inhibitory effect(25 mg/L) at 72 h. Conclusion:DOP exhibited toxicity against 3rd instar larvae of P. rapae L. AChE is not target of deoxypodophyllotoxin and ATPases may be one important target of deoxypodophyllotoxin.
ZHANG Shou-Gang,LIU Yan-Qing,GAO Rong,HE Bin,Zhao Hong-yan,DI Xu-Dong,CHENG Jie,TIAN Xuan,XIAO Hang.Effects of deoxypodophyllotoxin on the activity of AchE and ATPase in the head of 3rd instar larvae of Pieris rapae L.[J].,2007,(6):542-545.