Objective:To clone and analyze the different splicing variant of Lims gene in mouse. Methods:Splicing variants of Lims gene were amplified by RT-PCR from mouse cDNA and inserted into PinPointTM Xa-1T vector, two position clones selected by PCR were sequenced. Results:Lism E, a novel splicing variant with a 1164 bp open reading frame(ORF), encoding a 387-amino acid(AA) protein was cloned. Conclusion:Comparative genome analysis displayed Lims E, a novel variant of Lims gene was confirmed in mouse and established the foundation for further to studying the function of Lims E in cell development.
CHENG Li-bo,CHEN Jie,ZHAO Mu-zi,LIU Qing-huai,LI Jian-min.Lims E:molecular cloning and characterization of a novel splice variant of the LIM domain-family gene[J].,2007,(6):550-554.