Objective:To clone and analyze the different splicing variant of Lims gene in mouse. Methods:Splicing variants of Lims gene were amplified by RT-PCR from mouse cDNA and inserted into PinPointTM Xa-1T vector, two position clones selected by PCR were sequenced. Results:Lism E, a novel splicing variant with a 1164 bp open reading frame(ORF), encoding a 387-amino acid(AA) protein was cloned. Conclusion:Comparative genome analysis displayed Lims E, a novel variant of Lims gene was confirmed in mouse and established the foundation for further to studying the function of Lims E in cell development.
程立波,陈 洁,赵沐子,刘庆淮,李建民.Lims E:一个新的LIM同源域基因的克隆和初步分析[J].南京医科大学学报(自然科学版英文版),2007,(6):550-554.