Objective:To study nuclear factor-κB(NF-κB) activation in heart failure due to chronic pressure-overload. Methods: SD rats were performed by transverse aortic banding for 12 weeks. After that hemodynamic parameters and cardiac fibrosis were measured;NF-κB binding activity was analyzed by electrophoretic mobility shift assay(EMSA);Immunoprecipitation(IP) was performed to analysis Toll-like receptor 4(TLR4) and myeloid differentiation primary-response protein 8(MyD88) association. Results:Both systolic and diastolic function were significantly reduced compared with sham operated group(P < 0.05), and cardiac fibrosis enhanced predominantly. NF-κB binding activity in hypertrophic heart tissue was higher than that of sham operated group(P < 0.05) as well as TLR4/MyD88 association(P < 0.05). Conclusion:NF-κB was required for chronic pressure overload-induced heart failure.