Objective:To study the protein expression about the HIF-1α,ERK and p-ERK on ischemic myocardium after acute myocardiac infarction(AMI) and to explore initially the mechanism about HIF-1α. Methods:Rabbits AMI model were establised, and divided into 4 groups at random, and infected with Ad-HIF-1α,Ad-Blank,Rz-HIF-1α respectively. Sham group was regarded as control. The protein expression of HIF-1α,ERK and p-ERK was detected by immunohistochemistry and Western-blot. Results:The protein expression of exogenous HIF-1α and p-ERK increased at 1d after AMI, reached the peak at 7d and decreased gradually at 14d and 28d. At 56d,the protein of HIF-1α and p-ERK was not expressed. Among four groups, there was no difference in the protein level of ERK,while p-ERK protein expression was higher in group Ad-Blank than that in group Sham, lower than that in group Ad-HIF-1α,and the protein level of p-ERK was the lowest in group Rz-HIF-1α. Conclusion:Ad-HIF-1α was transducted effectively and promote ERK phosphorylated. HIF-1α ribozyme gene can block the protective effect of HIF-1α on the ischemic myocardium.