Objective:To explore the expression of osteopontin(OPN), CD44v6 in human pancreatic carcinomas and analyze the relationship between their expression and clinicopathologic features. Methods:The expression of OPN,CD44v6 was tested by immunohistochemistry in 43 cases of pancreatic carcinomas,34 cases of pancreatic paraneoplastic tissues. Results:In 43 cases of pancreatic carcinomas, the expression rates of OPN,CD44v6 were 65.1%,62.7% respectively;in 34 cases of pancreatic paraneoplastic tissues, the expression rates of OPN and CD44v6 were 20.6% both. OPN and CD44v6 expression was higher in carcinomas with lymph node metastases than that without metastases; CD44v6 expression was higher in carcinomas with histological grade Ⅲ(P < 0.05) than that with Ⅰ~Ⅱ grade, and there were strong correlation between OPN and CD44v6(r = 0.446). Conclusion:The expressions of OPN and CD44v6 were higher in pancreatic carcinomas. They might play an important role in the progression of human pancreatic carcinomas and participat in the invasion and metastasis of pancreatic carcinomas.