Objective:To establish lung perfusion model of rabbit in vivo. and to investigate the isolated lung preservation effect of the perfusion fluid blended with different compositions. Methods:30 rabbits were randomly divided into 5 groups which were control group(A), UW fluid with glyceryltrinitrate group(B), UW fluid with PGE group(C), UW fluid blended with auto-artery-blood (1:2) group(D) and UW fluid blended with auto-artery-blood (1:4) group(E). The lung and heart of rabbit were Dissociated as the isolated lung and heart model. the model were Perfused with low temperature perfusion fluid and conserved in the same temperature perfusion fluid. The lung specimens were obtained in order to analyze the change in morphology by using electron microscope at 2 and 4 hours after perfusion. the ratio of wet/dry weight(W/D), airway pressure(AWP) of lung model Detectd; were the blood which was pumped into the lung model through main pulmonary artery and then was elicited from atrium pulmonale by using a vacuum aspiration tube were detected after 4 hours of perfusion. Results:The difference of protection to the function of lung tissue gas exchange in E and D group was not remarkable, The protective effect to the function of the lung tissue gas exchange in E and D group surpasses the protective effect of other group significantly. There was no notable difference on the protective effect to the function of lung tissue gas exchange between B and C group. But the result of B and C group shows more superiority to group A. Conclusion:The experimental model was good in reliability and constancy. The group adding auto-artery-blood to the UW perfusion fluid can get longer time of preservation and better preservation effect of the function of lung tissue gas exchange than other group. Compared with the pure UW perfusion fluid group, the superiority of the preservation effect of perfusion fluid with glyceryltrinitrate and PGE to the function of lung is demonstrated again. But the preservation effect of the perfusion fluid with glyceryltrinitrate and PGE appeared inferior to that of the perfusion blended with auto-artery-blood.