Objective:To evaluate the correlation between the expression of Pin1 and β-catenin. To identify whether Pin1 was a prognostic factor in colorectal cancer. Methods:The expression of Pin1 and β-catenin proteins was detected by immunohistochemistry in 50 cases of colorectal cancer tissues. The relationships between Pin1 and β-catenin expression and clinicopathological factors were evaluated. Results:The overexpression of Pin1 and the aberrant accumulation of β-catenin in colorectal cancer was 36%(18/50) and 58%(29/50),respectively. A significant positive correlation was found between Pin1 and β-catenin(P = 0.006). The overexpression of Pin1 was related to pathological grade and tumor stage(P < 0.05). Pin1 was significantly related to the overall survival(P < 0.01). Patients with negative β-catenin expression survived longer than those with aberrant expression despite that no statistic significance was detected(P = 0.099). Conclusion:Pin1 was overexpressed and β-catenin was aberrant accumulated in colorectal cancer in this study. There was a significant correlation between their expression. Pin1 maybe a prognostic factor in colorectal cancer.