Objective：To investigate the function of promoter hypermethylation of secreted frizzled-related protein 2（SFRP2） gene in colorectal cancer. Methods：The promoter hypermethylation of SFRP2 gene in 44 sporadic colorectal cancer tissues and 20 adjacent normal mucosas of colorectal cancers were detected by fluorescence-based real-time PCR assay（MethyLight）， using 20 colorectal tissues of healthy volunteer as controls. And the relationship between hypermethylation of SFRP2 gene and clinicopathological features were analyzed. Results：SFRP2 gene hypermethylation was found in 81.8%（36／44）colorectal cancer tissues and 40%（8／20）adjacent normal mucosas of colorectal cancer. None of the normal colorectal mucosa tissues of volunteers showed any hypermethylated bands of SFRP2. Methylation in colorectal tumors was more frequent than in normal mucosa and adjacent tumor normal mucosa. Moreover，no significant associations were observed between SFRP2 hypermethylation and clinicopathological features such as sex，age， tumor stage，location and histological grade. Conclusion：Hypermethylation of SFRP2 gene is associated with the development of colorectal cancer. Hypermethylation of SFRP2 gene might serve as a biomarker for colorectal cancer screening.