Generation and cytotoxic analysis of PADRE/MUC4 specific CTL elicited by DC infected with recombinant adenovirus in vitro
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    Abstract:

    Objective:To discuss the cytotoxic activity of CTL,stimulated by adenovirus containing the universal DR-restricted Th helper epitope(PADRE) combined with HLA-A1 and HLA-A2 restricted epitopes from mucin4 gene(PADRE/MUC4) infected DCs. Methods:Recombinant adenovirus with PADRE/MUC4 gene was prepared,DCs of HLA-A2 positive donors were induced from PBMC in vitro. PBMC primed with adenovirus infected DC for 3 weeks,the specific cytotoxic activity of CTL was detected by standard 4 h Cr51 release assay and Elispot method. Results:Lymphocytes primed with pAd-CMV-PADRE/MUC4 caused potent cytotoxic responses. By contrast,lymphocytes primed with a GFP expressing adenovirus or mock-infected DCs had no cytotoxic effect. Conclusion:Infection of DCs with an adenovirus encoding PADRE combined with epitopes from mucin4 gene may be one possible strategy for immunotherapy of MUC4-expressing tumors.

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吴峻立,卫积书,孟 凯,高文涛,陈建敏,张静静,朱 毅,苗 毅. PADRE/MUC4 重组腺病毒转染树突状细胞诱导特异性CTL体外杀伤作用研究[J].南京医科大学学报(自然科学版英文版),2007,(12):1349-1352.

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  • Received:October 18,2007
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